Fig. 2.

Hypoxia promotes primary sarcoma migration. (A) Light microscope (Left) and fluorescence microscope (Right) images of day 3 images of KIA-GFP sarcoma tumors encapsulated within nonhypoxic and hypoxic matrices. Migrating GFP cells were tracked to determine 3D trajectories of tracked cells (representative trajectories) (scale bars: 100 μm) (B); overall speed (C); and MSD in the x, y, and z directions (D). Plots were created using the position of KIA-GFP cells in the hydrogels. Significance levels: *P < 0.05; ^{^}P < 0.01; ^#P < 0.001.