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. 2016 Aug 3;113(33):E4828–E4836. doi: 10.1073/pnas.1609792113

Fig. 5.

Fig. 5.

RNAi of Homeobox protein Six4 rescues miR-309 depletion phenotype. Average follicle size of ovaries (A) and egg numbers deposited per female individual (B) were analyzed from WT; dsSix4/Ant-Con, coinjection of Six4 dsRNA and control antagomir; dsSix4/Ant-309, coinjection of Six4 dsRNA and antagomir of miR-309; dsLuc/Ant-309, coinjection of Luciferase dsRNA and antagomir of miR-309; and Ant-309 injected female mosquitoes. (C–F) The expression profiles of four genes, including SIX4 (AAEL010327), FOXL (AAEL005741), Bruno (AAEL000695), and Aubergine (AAEL013692), were quantified in different experimental groups, as above. RE used on y axis is relative expression to ribosomal protein S7 (RPS7). Data represent six biological replicates with 8–10 individuals in each replication and are shown as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.