Fig. 3.

SLAMF3 coengagement promotes the activation of the IL-2/IL-2R/pSTAT5 pathway. Human naïve CD4⁺ T cells were stimulated for 18 h, after which expression of pSTAT5 was assessed by Western blot analysis and flow cytometry. (A) Representative blot and cumulative densitometry ratio of pSTAT5 over total STAT5. Data are mean ± SEM; n = 4. (B) Representative flow panels of pSTAT5 [top number, frequency (%); bottom number, MFI] and cumulative data (mean ± SEM); n = 17. (C) Naïve CD4⁺ T cells were stimulated for 18 h in the presence of an anti–IL-2 neutralizing antibody (2 μg/mL). Data are representative of 6–10 subjects per group (mean ± SEM). (D) Intracellular cytokine staining showing IL-2 production by naïve CD4⁺ T cells after 18 h of stimulation. Shown are representative flow panels and cumulative data (mean ± SEM); n = 9.