Fig. S5.

Anti-SLAMF3 antibody specifically binds to SLAMF3 on the cell surface. The CRISPR/Cas9 system was used to knock out SLAMF3 expression in Jurkat cells. (A) The expression of SLAMF3 (MFI) was assessed by flow cytometry after SLAMF3 Cas9 RNP treatment or control Cas9 RNP treatment. (B) SLAMF3/control Cas9 RNP-treated cells were stimulated with anti-SLAMF3 and/or isotype control (ISO) and/or anti-CD3 mAbs. After 18 h, CD25 expression (MFI and % of CD25-expressing cells) was measured by flow cytometry. Shown are the cumulative results of four independent experiments. Data are mean ± SEM.