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CD4+ T helper polarizing conditions
| Polarization | Cytokines | Neutralizing antibodies | ||
| Concentration | Concentration | |||
| Th1 | IL-12 | 20 ng/mL | Anti–IL-4 | 5 μg/mL |
| Th2 | IL-4 | 50 ng/mL | Anti-IFNγ | 5 μg/mL |
| Th17 | IL-1β | 25 ng/mL | Anti–IL-4 | 5 μg/mL |
| IL-6 | 50 ng/mL | Anti-IFNγ | 5 μg/mL | |
| IL-23 | 50 ng/mL | |||
| TGFβ1 | 2 ng/mL | |||
| Treg | IL-2 | 50 UI/mL | Anti–IL-4 | 5 μg/mL |
| TGFβ1 | 5 ng/mL | Anti-IFNγ | 5 μg/mL | |
