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. 2016 Aug 1;113(33):9321–9326. doi: 10.1073/pnas.1605081113

Table S3.

CD4+ T helper polarizing conditions

Polarization Cytokines Neutralizing antibodies
Concentration Concentration
 Th1  IL-12 20 ng/mL Anti–IL-4 5 μg/mL
 Th2  IL-4 50 ng/mL Anti-IFNγ 5 μg/mL
 Th17  IL-1β 25 ng/mL Anti–IL-4 5 μg/mL
 IL-6 50 ng/mL Anti-IFNγ 5 μg/mL
 IL-23 50 ng/mL
 TGFβ1 2 ng/mL
 Treg  IL-2 50 UI/mL Anti–IL-4 5 μg/mL
 TGFβ1 5 ng/mL Anti-IFNγ 5 μg/mL