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Incorporation of EFdA-MP against a matched template base (dT-MP), followed by a misincorporation against a mismatched template base (dA-MP). (A) Primer extension reactions at different EFdA-TP concentrations. (B) Sequence of the template/primer (5′-Cy3-DNAd20/DNAd27) used in the primer extension reactions and in the crystal structure of RT/DNAEFdA-MPP•EFdA-MP*N. EFdA-MP was incorporated as a match in the first site (+1 in blue) and as a mismatch in the following site (+2, in red).
