Fig. S1.

GBA does not bind directly to Hsp90 clients and cochaperones. HEK293 cells were transfected with control siRNA or Hsp90β siRNA (20 nM). Cell lysates were prepared after 72 h and incubated with biotinylated GBA to isolate Hsp90–client/cochaperone complexes. “Input” lanes reflect lysate concentrations of Hsp90β and each client or cochaperone. Biotinylated GBA pulldown lanes are shown (Left). Although cochaperone and client expression was not affected by silencing Hsp90β, copulldown of these proteins was reduced, consistent with the degree of reduction in expression of Hsp90β following siRNA silencing.