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. 2016 Jul 27;113(33):E4801–E4809. doi: 10.1073/pnas.1606655113

Fig. S2.

Fig. S2.

Biotinylated GBA and STA-7346 (biotinylated N-terminal domain inhibitor) binding to Hsp90β truncation mutants. HEK293 cells were transfected with various 3×F-Hsp90β truncation mutants. After cell lysis, biotinylated GBA and STA-7346 were added to isolate 3×F-Hsp90β. (A–C) C-terminal truncations extending into the MD beyond amino acid 432 in Hsp90β are not recognized by biotinylated GBA, whereas STA-7346 bound all C-terminal truncation mutants retaining the NTD. (D) Densitometric quantification of selected data obtained in C, normalized for input. IP, immunoprecipitation. (E) N-terminally truncated Hsp90β (C268–648) retains binding to GBA but not to STA-7346.