Table 1.

Oligonucleotide primer sequences for quantitative reverse transcriptase polymerase chain reaction

Gene	Primer sequence (forward)	Primer sequence (reverse)	Annealing temperature (°C)	Product size (bp)
COX-2	5-TTGCTGGCAGGGTTGCTGGT-3’	5-TTGCTGGCAGGGTTGCTGGT-3’	60	86
TNF-a	5-GAGGCCAAGCCCTGGWG-3’	5-CGGGCCGATTGATCTCAGC-3’	60	91
MUC5AC	5-CAGCACMCCCCTGTTTCAM-3’	5’-GCGCACAGAGGATGACAGT-3’	60	99
MUC5b	5-GCCTACGAGGACTTCAACGT-3’	5’-CCTTGATGACCACACGGGTG-3’	60	79
TP53	5’-AGACCTGTGGGAAGCGAAAA-3’	5-TCATCCATTGCTTGGGACGG-3’	60	106
BAX	5’-CCAAGGTGCCGGAACTGA-3’	5’-CCCGGAGGAAGTCCAATGT-3’	60	57
BCL-2	5-TCCCTCGCTGCACAAATACTC-3’	5’-ACGACCCGATGGCCATAGA-3’	60	72
NOX4	5-CTCAGCGGMTCAATCAGCTGTG-3’	5-AGGAACACGACAATCAGCCTTA-3’	60	284
SOD1	5’-GTAATGGACCAGTGAAGGTGTG-3’	5’-CAATTACACCACAAGCCAAACG-3’	60	375
GAPDH	5-TCGCCCCACTTGATTTTGG-3’	5’-GCAAATTCCATGGCACCGT-3’	60	105