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. 2016 Jul 26;11(15):1971–1991. doi: 10.2217/nnm-2016-0128

Figure 2. . Plasmid DNA loading efficiency of LGA-PEI polymer and particle size distribution.

Figure 2. 

LGA-PEI (0.5:1 w/w) polymer was used. (A) DNA gel retardation assay was performed on 0.8% agarose gels containing 25 nM ethidium bromide. Different amounts of LGA-PEI polymer (0, 5, 10, 15, 20, 25 or 30 μg) were mixed with 10 μg plasmid DNA. (B) Optical measurement. Free DNA or polymer/DNA NPs suspended in solution were detected with spectrophotometry. Plasmid DNA (10 μg) was mixed with 0 to 30 μg LGA-PEI polymer and centrifuged. (C) Size distribution of 25 μg LGA-PEI/10 μg plasmid DNA (double-stranded, circular DNA) NPs. The size of the particles in solution was determined with a Zeta Potential Analyzer. (D) Size distribution of 25 μg LGA-PEI/15 μg oligonucleotide DNA (single-stranded, 23 nucleotides).