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. 2016 Jul 26;11(15):1971–1991. doi: 10.2217/nnm-2016-0128

Figure 3. . Direct observation of LGA-PEI/DNA NPs with scanning electron microscopy and atomic force microscopy.

Figure 3. 

LGA-PEI polymer with 64% PEI corresponding to the LGA-PEI 0.5:1 w/w polymer was used. (A–C) are SEM images. (D) Average particle size of three different LGA-PEI/DNA N/P ratios (6, 10 and 14) in SEM images. LGA-PEI/DNA N/P ratios (6, 10 and 14) are correspondent to its w/w ratios (1.5:1, 2.5:1 and 3.5:1), respectively. (E–G) are AFM images of samples prepared with LGA-PEI polymers (64% PEI) and plasmid DNA (4.7 kb) at nitrogen/phosphorus (N/P) ratios of 6, 10 and 10. (H) Control: 15 μg PEI and 10 μg plasmid DNA. (I) Control: 35 μg LGA-PEI polymers and 10 μg modified mRNA of GFP.

LGA-PEI: Lactic-co-glycolic acid-modified polyethylenimine; NP: Nanoparticle; PEI: Polyethylenimine.