Skip to main content
. 2016 Jul 26;11(15):1971–1991. doi: 10.2217/nnm-2016-0128

Figure 4. . Cytotoxicity of LGA-PEI/plasmid DNA NPs in vitro.

Figure 4. 

LGA-PEI (0.5:1 w/w) polymer was used. (A) Comparison between LGA-PEI polymer and unmodified PEI. Different amounts of LGA-PEI polymer were mixed with plasmid DNA (1 μg/well in 96-well plates). The amount of PEI in LGA-PEI and unmodified PEI was the same. Pancreatic cancer cell line (PANC-1) cells were incubated overnight with nanoparticles in 10% FBS DMEM medium; cell viability was determined with the MTT assay. n = 3/group. (B) Comparison between LGA-PEI polymer and commercial lipofectamine 2000. NPs of LGA-PEI/DNA (2.5/1) or lipofectamine 2000 delivered different amounts of plasmid DNA into PANC-1 cells.

LGA-PEI: Lactic-co-glycolic acid-modified polyethylenimine; PEI: Polyethylenimine.