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. 2016 Jul 26;11(15):1971–1991. doi: 10.2217/nnm-2016-0128

Figure 5. . Gene delivery efficiency of LGA-PEI/DNA or RNA NPs in vitro determined by immunofluorescence.

Figure 5. 

LGA-PEI (0.5:1 w/w) polymer was used. (A) Delivery of plasmid DNA to pancreatic cancer cell line (PANC-1). LGA-PEI/plasmid DNA (0.2 μg/well in 96-well plates), and lipofectamine 2000/DNA or PEI/DNA controls containing GFP gene, were incubated with PANC-1 cells for 20 h. (B) Delivery of GFP gene-containing plasmid DNA to a cholangiocarcinoma cell line (M213). LGA-PEI polymers or lipofectamine 2000 control with GFP gene-containing plasmid (4 μg/well in the 6-well plate) were incubated with M213 cells for 48 h. (C) Delivery of DNA into THP-1 (suspension cell culture). LGA-PEI or lipofectamine 2000 delivered 2.5 μg DNA/well in 12-well plates for 48 h. (D) Human fibroblasts (BJ). LGA-PEI and lipofectamine 2000 delivered 1 μg DNA/well in 12-well plates for 48 h. (E) Delivery of modified GFP mRNA (mmRNA) into PANC-1 cells (10 μg RNA/well in 12-well plates) in 48 h. (F) Delivery of synthetic miR-520h mimic labeled with DY-547 (1 μg double stranded RNA/well in 8-well chamber slides) by LGA-PEI or lipofectamine 2000 into PANC-1 cells in 24 h. Control: miR-520h mimic alone. (G) Fluorescence density analysis of delivery of synthetic miR-520h mimic labeled with DY-547 (1 μg double-stranded RNA/well in 8-well chamber slides) into PANC-1 cells in 24 h by LGA-PEI copolymer and lipofectamine 2000.

LGA-PEI: Lactic-co-glycolic acid-modified polyethylenimine; PEI: Polyethylenimine.