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. 2016 Aug 8;5:59–71. doi: 10.2147/OV.S108831

Figure 1.

Figure 1

Effects of incubating cell cultures with MYXV-red (moi =0.1).

Notes: (A) Cytopathic effects: forty-eight hours after inoculation with MYXV-red, red fluorescence is observed in nearly all RK13 (fully permissive) cells and in lower numbers of CCL-136 cells, but not in fibrocytes (nonpermissive) cells. Magnification 1000x. (B) Viral growth curves: MYXV-red multistep growth kinetics in RK13 and CCL-136 cells is shown. Data are expressed as the logarithm of viral plaque-forming units per milliliter (pfu/mL) of infected cell lysate over time and depict the average of three experiments. Error bars indicate standard error of the mean. (C) Cell viability: viability of RK13 and CCL-136 cells was determined 72 hours postinoculation with MYXV (moi =1). Data are presented as the percentage of the average fluorescence intensity of mock-infected cells. Error bars indicate standard deviation (n=6). *P-values of mock versus MYXV-infected cells ≤0.0004.

Abbreviations: MYXV-red, myxoma virus with red fluorescent protein; moi, multiplicity of infection; RK13, rabbit kidney epithelial cells; CCL-136, human rhabdomycosarcoma cells.