Table 1.

Primer sequences and specific PCR parameters used to detect MYXV-red DNA in tissues

Target gene segment(s)	Forward primer sequence	Reverse primer sequence	Annealing temperature (°C)	Product length (base pairs)
MYXV M034L (DNA polymerase)	5′ CTG CGT AGA CGA CGG C 3′	5′ CGG AGG ATT TGG TGT CGG 3′	60	600
MYXV M033R and M034L	5′ CAC CCT CTT TAG TAA AGT ATA CAC C 3′	5′ GAA ATG TTG TCG GAC GGG 3′	56	800
MYXV M151R (serp2)	5′ GGC GAC TGG AGA CTG G 3′	5′ CCG CGT ACG GGG GAC TG 3′	60	950

Note: Three partial gene segments were amplified for each tissue sample.

Abbreviations: PCR, polymerase chain reaction; MYXV, myxoma virus; MYXV-red, MYXV with red fluorescent protein.