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. Author manuscript; available in PMC: 2016 Aug 24.
Published in final edited form as: New Phytol. 2013 May 15;199(3):787–799. doi: 10.1111/nph.12312

Fig. 1. Silencing MPK4 specifically elevates simulated M. sexta herbivory-induced JA and JA-Ile levels.

Fig. 1

Wild-type (WT) and irMPK4 plants (line 119 and 163) were wounded with a pattern wheel and 20 μL of water (W+W) or M. sexta OS (W+OSMs) were immediately applied to wounds. Samples were harvested at indicated times, and their JA (a) and JA-Ile (b) contents (mean ± SE) were analyzed with HPLC-MS/MS. Asterisks indicate significant differences between WT and irMPK4 plants (t-test; *, P < 0.05; **, P < 0.01; N = 5).