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. Author manuscript; available in PMC: 2016 Aug 24.
Published in final edited form as: New Phytol. 2013 May 15;199(3):787–799. doi: 10.1111/nph.12312

Fig. 2. Simulated M. sexta feeding does not induce large changes of known defensive metabolites.

Fig. 2

(a) M. sexta growth on different plants. Wild-type (WT) and irMPK4 plants (lines 119 and 163) were infested with 30 neonate M. sexta larvae (1 larva/plant). Masses of these larvae (mean ± SE) on WT and irMPK4 plants were recorded on day 4, 7, 9, and 11. (b to e) Accumulations of defensive secondary metabolites in WT and irMPK4 plants after wounding and simulated herbivory. WT and irMPK4 plants were wounded with a pattern wheel and 20 μL of water (W+W) or M. sexta OSMs (W+OSMs) were immediately applied to the puncture wounds. The activity of TPI (b) and contents of nicotine (c), caffeoylputrescine (d), and 17-hydroxygeranyllinalool diterpene glucosides (HGL-DTGs) (e) (mean ± SE) were analyzed in samples harvested 3 days after treatment. Asterisks indicate significant differences between the masses of larvae reared on WT and irMPK4 plants [t-test; *, P < 0.05; ***, P < 0.001; N = 30 for (a) and N=5 for (b to e)].