Skip to main content
. 2016 Aug 22;240(1):61–71. doi: 10.1002/path.4754

Figure 6.

PATH-4754-FIG-0006-c

(A) Flow cytometry of isolated human neutrophils showing C5 expression, without and with cell permeabilization, in unstimulated conditions, and after priming with TNFα followed by stimulation with monoclonal anti‐MPO, anti‐PR3 or fMLP. Numbers shown are median fluorescence intensity. (B) Data under the same conditions are shown for C5a staining. Similar results (to panels A and B) were obtained in an experiment from a different neutrophil donor. (C, D) Histological readouts of glomerular CD68+ macrophages and glomerular crescents in anti‐MPO vasculitis in four groups of bone marrow chimaeric mice constructed with wild‐type or C5‐deficient donors and recipients. (E–G) Functional readouts of haematuria, albuminuria, and serum creatinine in the same experiment. For albuminuria and serum creatinine, baseline data from the same animals are shown. Each symbol represents a separate mouse. WT = wild type, C5 Def = C5 deficient. + p < 0.05; ++ p < 0.01; +++ p < 0.001. (H) Representative renal histology showing periodic acid Schiff (PAS)‐stained sections and immunofluorescence staining for CD68+ macrophages from mice in this experiment. Scale bars = 20 µm. Error bars are mean ± SEM.