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. Author manuscript; available in PMC: 2016 Aug 24.
Published in final edited form as: J Pineal Res. 2015 Oct 20;60(1):3–15. doi: 10.1111/jpi.12270

Fig. 4.

Fig. 4

Lung metastasis after 2 and 5 wk of melatonin and Y27632 treatments. Semiquantitative analysis of radioactivity intensity by single photon emission computed tomography (SPECT) images following (A) 2 wk of treatment and following (C) 5 wk of treatment. SPECT images obtained after injection of the 99mTc-tetrofosmin showing lung metastasis following (B) 2 wk of treatment and following (D) 5 wk of treatment. (a) and (b) negative control, (c) and (d) positive control, (e) and (f) melatonin treatment, (g) and (h) Rho-associated kinase protein (ROCK)-1 inhibitor (Y27632) treatment. (E) High-resolution computed tomography images at 25-µm thickness. (a) Control animal without metastasis; (b) positive control animal without treatment showing high-density area (white arrow) indicating lung metastasis in animal after 6 wk following induction of lung metastasis. Significant value in ANOVA followed by Bonferroni’s test (±S.E.M. *P < 0.05; ***P < 0.0001).