Fig. 7.
Rho-associated kinase protein (ROCK)-1 protein expression analysis in lung metastasis. (A) and (C) Semiquantitative analysis from animals with 2 and 5 wk of treatment, respectively. (B) and (D) Representative images from (a) and (b) negative control, (c) and (d) positive control, (e) and (f) melatonin treatment, (g) and (h) Y27632 treatment. Images were taken with 10× and 40× magnification. Arrows show the sites of metastasis. The values of mean optical density and standard error for each group are shown in arbitrary units (au). (*) represents P ≤ 0.05 and (**) P ≤ 0.001 by ANOVA followed by Bonferroni’s test.