Table 5.
Detection Method | Solid Support | Assay Format | Analyte | Sensitivity | Spots/Array (n × n) | Reference |
---|---|---|---|---|---|---|
Fluorescence | Silicon | Direct immunoassay | Guinea pig IgG | 6 ng/mL | 25 × 25 | [15] |
Plasmon | PDMS | Hybridization | RNA/DNA | [87] | ||
Fluorescence | Glass | Sandwich immunoassay | Bacteria | 6 × 6 | [88] | |
Fluorescence | PDMS | Direct immunoassay | Gp41; gp120 | [72] | ||
Fluorescence | PDMS | Sandwich immunoassay | Human TNF | 20 pg/mL | 10 × 17 | [73] |
Bioluminescence | PDMS | Intracellular signal | Cells | 5 × 5 | [89] | |
Fluorescence | PDMS | Sandwich immunoassay | C-reactive protein | 30 ng/mL | 7 × 7 | [90] |
Fluorescence | PDMS | Direct immunoassay | Multiple Bacterial antigens | 1 × 5 | [74] | |
Fluorescence | PDMS | Direct assay | Cells | 8 × 6 | [91] | |
Fluorescence | PDMS | Direct immunoassay | Antibodies against bacteria in serum | 6 × 6 | [92] | |
Fluorescence | Silicon nitride | Sandwich immunoassay | C-reactive protein | 2.5 µg/mL | 3 × 12 | [93] |
Fluorescence | PDMS | Quantum dot-based sandwich immunoassay | Carcinoma embryonic antigen | 500 fM | 4 × 8 | [94] |
Fluorescence | PDMS | Sandwich immunoassay | C-reactive protein | 1ng/mL | 5 × 14 | [78] |
Fluorescence | PDMS | Sandwich immunoassay | Oxidative stress biomarkers 3-nitro tyrosine, Catalase Superoxide dismutase | 150 µM 5 ng/mL 0.5 ng/mL |
3 × 10 | [95] |
Fluorescence | PVDF, PDMS | Direct immunoassay | 1 × 10 | [96] | ||
Fluorescence | PDMS | Direct immunoassay | IgG | 5 ng/mL | 1 × 4 | [79] |
Various | Various | Various | [97] (Patent number: US 8,075,854 B2) | |||
Fluorescence | Polycarbonate and PDMS | Sandwich immunoassay | Rabbit IgG | 0.16 µM | 6 × 5 | [77] |
Fluorescence | PDMS | Sandwich immunoassay | Panel of HIV associated antigens | 8 × 21 | [98] | |
Fluorescence | PDMS | Sandwich immunoassay | Panel of HIV associated antigens | [99] |