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. 2015 Feb 16;4(1):64–83. doi: 10.3390/microarrays4010064

Figure 4.

Figure 4

Primary human hepatocytes entrapped in rat-tail collagen combined with continuous flow in the µ-Slide VI 0.4 show a higher viability and glucose production. Cells were either seeded in 2D or 3D in 96-well plates (2.5 × 104 cells/well) or entrapped in collagen in the µ-Slide VI 0.4 (1.5 × 105 cells/µ-Slide VI 0.4) and cultivated for 1 day or 7 days. To evaluate cell viability, the cells were stained either with 2 μM Calcein AM to visualize living cells or with 4 μM ethidium homodimer to visualize dead cells, while the turnover of resazurin to resorufin was measured in parallel. The glucose production was measured from cell culture supernatants. (a) Live/dead staining of primary human hepatocytes on day one and day seven. Pictures were taken with an Evosfl microscope from Peqlab Biosystems (Erlangen, Germany); (b) Comparison of resazurin turnover and glucose production. Cell viability is depicted as % of viable cells compared to day one. The graphs were drawn with the GraphPad Prism software (GraphPad Software, version 5.01). The values are depicted as mean and standard deviation of three donors/experiments.