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. 2015 Jan 21;4(1):2–24. doi: 10.3390/microarrays4010002

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Experimental strategy for genome-wide analysis of the ischemic core (IC) and penumbra (P) transcriptome following pituitary adenylate-cyclase activating polypeptide (PACAP38) treatment. Diagrammatic representation of the permanent middle cerebral artery occlusion (PMCAO) model (left-hand side). Total RNA was extracted from the IC and P in the ipsilateral (right) hemisphere along with the corresponding regions in the healthy contralateral hemisphere. PACAP38 was the treatment and saline served as the control. DNA microarray analysis was performed using two oligo DNA chips (Agilent Technologies) as indicated using the two-color, dye-swap approach (right-hand side).