Skip to main content
. 2015 Nov 23;16(3):808–820. doi: 10.1111/ajt.13521

Figure 4.

Figure 4

Effect of recombinant lipocalin 2:siderophore:Fe complex (rLcn2) on the expression of endogenous lipocalin 2 (Lcn2) in the renal allografts.  Kidneys transplanted from C57Bl/6 (Bl/6) to Bl/6, from Bl/6 to Balb/c and from Balb/c to Bl/6 mice were harvested at posttransplant day 7, and the expression pattern of Lcn2 mRNA and protein in the grafts was determined by quantitative polymerase chain reaction (A) and by immunohistochemistry (B). Representative box plot for mRNA expression (A) and images of the Lcn2 stained (B) sections of kidneys harvested from sham‐operated mice (a), Bl/6 kidney isografts (b), Bl/6 allografts in Balb/c recipients (c), Bl/6 Lcn2−/− allografts in Balb/c recipients (d), and Balb/c allografts in Bl/6 WT (e) and Lcn2−/− (f) recipients and the Bl/6 WT recipients treated with rLcn2 (250 μg perioperatively) (g) or cyclosporine A (10 mg/kg body weight, daily) (h) are shown (n = 6). Scale bars = 100 μm. *p < 0.05, **p < 0.01.