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. 2016 Jun 30;5(9):1247–1256. doi: 10.5966/sctm.2015-0323

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Figure 4. — Bone marrow-derived mesenchymal stem cells infected with decorin-expressing adenovirus suppress hepatic fibrosis. (A, B): mRNA expression levels of collagen-1 (A) and α-SMA (B). (C, D): Representative western blot for α-SMA (C) and its densitometric analysis (D). Values are expressed as means ± SDs. ∗, p < .05, ∗∗, p < .01. (E): Immunofluorescence staining of α-SMA in liver tissue. The expression of α-SMA in the DCN-MSC-treated group (G4) was significantly decreased compared with the untreated cirrhotic group (G2) and the CA-MSC-treated group (G3). Nuclei were stained with DAPI. Merged immunofluorescence images of α-SMA (green) and DAPI (blue) are shown. Scale bar = 75 μm. Abbreviations: CA-MSC, control adenovirus-infected mesenchymal stem cell; DAPI, 4′,6-diamidino-2-phenylindole; DCN-MSC, decorin-expressing adenovirus-infected bone marrow-derived mesenchymal stem cell; mRNA, messenger RNA; α-SMA, α-smooth muscle actin.