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. 2016 Jun 30;5(9):1238–1246. doi: 10.5966/sctm.2015-0347

Figure 5.

Figure 5.

Donor BMMSCGFP survival and apoptosis in recipient bone marrow. (A–C): Representative images demonstrating Hoechst staining for total cells (blue), GFP immunofluorescence for donor BMMSCsGFP (green), TUNEL fluorescence for apoptosis (red), and merged double-labeling for apoptotic donor BMMSCsGFP (yellow). Mice were sacrificed at 24 hours after PBS or BMMSC infusion. Scale bars: 200 μm. (D, E): Corresponding parameters showing partial apoptosis of donor BMMSCsGFP with prevention of recipient bone marrow cell apoptosis. Data represent mean ± SEM; n = 4 per group. ∗∗∗, p < .001. Abbreviations: Ar, area; BMMSC, bone marrow-derived mesenchymal stem cell; Cont, control; GIOP, glucocorticoid-induced osteoporosis; GFP, green fluorescent protein; MSC, mesenchymal stem cell; ND, not detected; NS, not significant; PBS, phosphate-buffered saline; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.