Skip to main content
. 2016 Jul 7;5(9):1171–1181. doi: 10.5966/sctm.2015-0384

Figure 1.

Figure 1.

Characterization of control and FOCD-NS BM-MSCs. (A): Morphology of cultured mesenchymal cells isolated from control and FOCD-NS bone marrow. Scale bars, 200 µm. (B): Flow cytometric analysis of control and FOCD-NS BM-MSCs for positive antigens CD90, CD73, and CD105 and negative markers CD11b, CD34, CD19, CD45, and HLA-DR. White histograms represent isotype controls, and the gray overlay represents each antigen; percentage positive cells shown within histograms. (C): Adipogenic and osteogenic differentiation of control and FOCD-NS BM-MSCs. In adipogenic culture, phase-contrast microscopy images show Oil Red O-positive cells. Scale bars, 200 µm (left) and 100 µm (right). In osteogenic differentiation culture, mineralized nodules produced by osteoblasts were stained with Alizarin red. Scale bars, 100 µm. Abbreviations: APC, allophycocyanin; BM-MSC, bone marrow mesenchymal stem cell; Comp, compensated; Cy, cyanine; FITC, fluorescein isothiocyanate; FOCD-NS, familial osteochondritis dissecans from northern Sweden; HLA, human leukocyte antigen; PE, phycoerythrin; PerCP, peridinin chlorophyll.