Figure 2.

Morphologic and immunohistochemical analysis of in vitro chondrogenic differentiation using micropellet culture. Pellets were harvested at different time points (days 1, 3, 5, 7, and 14). (A): Representative stereomicroscope images of overall morphology of chondrogenic pellets differentiated from control and FOCD-NS BM-MSCs. Scale bars, 1 mm. (B): Toluidine blue staining on the sections of these pellets. Scale bars, 500 µm. (C): Representative images of immunostained sections for aggrecan distribution. Scale bars, 500 µm. Insets are magnified images of the areas indicated by black arrows. Abbreviations: BM-MSC, bone marrow mesenchymal stem cell; FOCD-NS, familial osteochondritis dissecans from northern Sweden.