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. 2016 Jun 30;5(9):1229–1237. doi: 10.5966/sctm.2015-0412

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Figure 7. — Mesenchymal stem cell (MSC) spheroids exhibit differences in matrix protein content compared with dissociated MSCs when entrapped in collagen hydrogels. Osteogenically induced adherent and spheroidal MSCs encapsulated within collagen I hydrogels were subjected to stimulus withdrawal (osteogenic media/growth media). Immunohistochemistry was performed at +5 days for Picrosirius Red (A, B), H&E (C, D), and type 1 collagen (E, F). Asterisks indicate areas of interest. Scale bar represents 500 µm. Abbreviations: Col1, Type 1 collagen; Diss., dissociated mesenchymal stem cells; PSR, Picrosirius Red; Sph., mesenchymal stem cell spheroids.