Table 3. Proteins whose production from pCA24N increased growth at low temperatures in the ΔdinJ ΔKmR strain as identified by screening the complete ASKA overexpression library.
Colony | Growth increase (%) | Protein | Function |
---|---|---|---|
1-3-57 | 32 | YhbU | U32 peptidase family protein. yhbUV divergent operon promoter region binds FNR and yhbUV operon expression is activated by FNR |
2-3-25 | 19 | SspA | Stringent starvation protein A, phage P1 late gene activator; RNAP-associated acid-resistance protein; inactive glutathione S-transferase homolog |
2-6-45 | 65 | Mlc (DgsA) | Global transcriptional repressor; regulates pts operon expression at P0; required for anaerobic growth on glucosamine, binds nagC promoters; regulates manX and malT; makes large colonies; autorepressor |
2-6-64 | 62 |