Table 2. Primary and secondary antibodies used in this study.
Primary antibodies | ||||
Target | Species | Dilution | Source | Reference |
CIRP | Mouse monoclonal | 1:300 | Proteintech | 60025-2-Ig |
RBM3 | Rabbit monoclonal | 1:1000 | Abcam | ab134946 |
Glutamine Synthetase | Mouse monoclonal | 1:300 | BD Bioscience | 610517 |
Calbindin D28K | Rabbit polyclonal | 1:150 | Santa Cruz Biotech. | sc-7691 |
Recoverin | Rabbit polyclonal | 1:150 | Santa Cruz Biotech. | sc-20353 |
Secondary antibodies | ||||
Specificity | Fluorochrome | Dilution | Source | Reference |
Donkey anti rabbit | Alexa Fluor 555 | 1:200 | Molecular Probes | A31572 |
Goat anti rabbit | Alexa Fluor 488 | 1:200 | Molecular Probes | A11008 |
Donkey anti mouse | Alexa Fluor 488 | 1:200 | Molecular Probes | A21202 |
Goat anti mouse | Texas Red | 1:200 | Molecular Probes | T-862 |