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. 2016 Aug 24;11(8):e0161458. doi: 10.1371/journal.pone.0161458

Table 2. Primary and secondary antibodies used in this study.

Primary antibodies
Target Species Dilution Source Reference
CIRP Mouse monoclonal 1:300 Proteintech 60025-2-Ig
RBM3 Rabbit monoclonal 1:1000 Abcam ab134946
Glutamine Synthetase Mouse monoclonal 1:300 BD Bioscience 610517
Calbindin D28K Rabbit polyclonal 1:150 Santa Cruz Biotech. sc-7691
Recoverin Rabbit polyclonal 1:150 Santa Cruz Biotech. sc-20353
Secondary antibodies
Specificity Fluorochrome Dilution Source Reference
Donkey anti rabbit Alexa Fluor 555 1:200 Molecular Probes A31572
Goat anti rabbit Alexa Fluor 488 1:200 Molecular Probes A11008
Donkey anti mouse Alexa Fluor 488 1:200 Molecular Probes A21202
Goat anti mouse Texas Red 1:200 Molecular Probes T-862