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. 2016 Aug 24;11(8):e0161877. doi: 10.1371/journal.pone.0161877

Fig 4. IL-31 regulates expression of genes involved in skin damage.

Fig 4

(A) C57BL/6 mice were injected intradermally with saline or rIL-31 (20μg) daily for 14 days. A portion of dorsal skin was excised and RNA was isolated. RNA-Seq analysis was performed using next-generation sequencing. Heat map shows two clusters of differentially expressed genes that were either up or down regulated (indicated with color key) in rIL-31-treated mice compared to saline-treated controls. A total of 1,016 significant gene results was analyzed using a P value cut-off of 0.05; FDR<0.1 and greater than two-fold changes. (B) Network representation of the biological-function enrichment analysis of IL-31-regulated genes identified by RNA-Seq and top-gene function analysis. Solid lines depict the interactions between the genes and biological processes.