Fig 4. MoFis1 and MoMdv1 are important for virulence in M. oryzae.

(A) Rice (Oryza sativa cv. CO39) seedlings were sprayed with conidial suspensions and examined 7 dpi. (B) Quantification of lesion types (per 1.5 cm²) on susceptible rice cultivar CO39 spayed with conidia of Guy11, ΔMofis1, ΔMomdv1, ΔMofis1/MoFIS1, and ΔMomdv1/MoMDV1 strains. Asterisks represent significant differences (Duncan's new multiple range test, p<0.01). (C, D) Detailed observation and statistical analysis for infectious growth in rice sheath cells at 36 hpi. Appressorium penetration sites (n = 100) were observed and invasive hyphae were rated from type 1 to 4. Error bars represent ±SD from three independent experiments. Bar = 5 μm. Asterisks indicate IH extended to surrounding cells. (E) Invasive hyphal growth of the ΔMofis1 and ΔMomdv1 mutants in rice sheath cells at 48 hpi. Asterisks indicate IH extended to surrounding cells.