Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 24;12(8):e1005831. doi: 10.1371/journal.ppat.1005831

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Ayres Pereira et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 2 — Fresh placental tissue was perfused with VAR2CSA-expressing parasites before biopsies were collected and stained. A) Bright-field of placental tissue. The region delineated by a black dotted square is shown in (B). Scale bar represents 20 μm. (B) Full-length VAR2CSA (FV2) immunostaining show expression of placental CS in the villous stroma (arrow heads) and in the apical membrane of the syncytiotrophoblast (arrows). The image is a composite of three distinct channels: blue (nuclei), green (placental CS stained by FV2), and red (placenta auto-fluorescence). Scale bar represents 20 μm. (C) FV2 immunostaining showing the expression of placental CS in mesh-like structures (arrows). The image is a composite of three distinct channels: blue (nuclei), green (placental CS stained with FV2), and red (placenta auto-fluorescence). Scale bar represents 20 μm.(D) Addition of soluble CSA (sCSA) out-competes FV2 binding to placental tissue. The image is a composite of three distinct channels: blue (nuclei), green (placental CS stained with FV2), and red (placenta auto-fluorescence). Scale bar represents 20 μm. (E) Bright-field of placental tissue. The region delineated by a black dotted square is shown in (F). Scale bar represents 20 μm. (F) Recombinant VAR2CSA (rVAR2) immunostaining show the expression of placental CS in the villous stroma (arrow heads) and in the apical membrane of the syncytiotrophoblast (arrows). The image is a composite of three distinct channels: blue (nuclei), green (placental CS stained with rVAR2), and red (placenta auto-fluorescence). Scale bar represents 20 μm. (G) rVAR2 immunostaining show expression of placental CS in mesh-like structures (arrows). The image is a composite of three distinct channels: blue (nuclei), green (placental CS stained with rVAR2), and red (placenta auto-fluorescence). Scale bar represents 20 μm.(H) Addition of soluble CSA (sCSA) out-competes rVAR2 binding. The image is a composite of three distinct channels: blue (nuclei), green (placental CS stained with rVAR2), and red (placenta auto-fluorescence). Scale bar represents 20 μm. (I) Bright-field of placental tissue. A region delineated by a black dotted square is shown in (I). Scale bar represents 20 μm. (J) Anti-CS-56 antibody (α-CS-56 ab) immunostaining show the expression of CS in the villous stroma (arrow heads) and in the apical membrane of the syncytiotrophoblast (arrows). The image is a composite of three distinct channels: blue (nuclei), green (CS stained with α-CS-56 ab), and red (placenta auto-fluorescence). Scale bar represents 20 μm. (K) α-CS-56 ab immunostaining show the expression of CS in mesh-like structures (arrows). The image is a composite of three distinct channels: blue (nuclei), green (CS stained with α-CS-56 ab), and red (placenta auto-fluorescence). Scale bar represents 20 μm.m(L) Addition of soluble CSA (sCSA) out-competes α-CS-56 ab binding. The image is a composite of three distinct channels: blue (nuclei), green (CS stained with α-CS-56 ab), and red (placenta auto-fluorescence). Scale bar represents 20 μm.