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. 2016 Jan 4;35(32):4225–4234. doi: 10.1038/onc.2015.487

Figure 6.

Figure 6

(a) Breast cancer cell lines were transfected with MUCL1 siRNA or NT control siRNA. Cell proliferation was assessed by Cell Titer Glo Assay each day for 1 week. Mean ±s.d. is shown (n=10). *P<0.001 for difference of growth rate. MUCL1 knockdown was confirmed by western blotting. (b) BT474 and KPL4 cells were transfected with MUCL1 or NT siRNA for 96 h, methanol-fixed and stained with propidium iodide for cell cycle analysis. The percent of cells in each cell cycle phase is shown as the mean ±s.d. (n=3). (c) Western blots of cell cycle regulators 72 h post transfection shows significant decreases in cyclins D1 and D3, as well as increases in the cyclin-dependent kinase (Cdk) inhibitors p21cip1 and p27kip1. The experiments were repeated twice with similar results.