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. 2016 Sep;90(3):555–567. doi: 10.1016/j.kint.2016.03.020

Figure S2.

Figure S2

Immunolocalization of β3-AR in mouse kidney. Paraffin-embedded kidney sections (C57BL6/J, wt) were immunostained with anti β3-AR antibodies (green) and co-stained with antibodies against specific markers of different segments of the kidney tubule or vasculature: AQP1 for the proximal tubule (PT) and the thin descending limb (TDL), AQP2 for the inner medullary collecting duct (IMCD) and CD31 for the endothelium of Vasa Recta (all in red). Overlay of the each double staining experiment indicated that β3-AR was neither expressed in the PT nor in the TDL nor in the IMCD nor in the Vasa Recta. Drawings of the nephron on the right column indicated in red the β3-AR-negative tubule or vascular portions. β3-AR was expressed at the basolateral plasma membrane in all the tubules where it is expressed. Same results were obtained in at least 5 animals (bar = 20 μm).