Fig. 2. Sharpened asymmetrically formed A-particle map.

(A) The sharpened A-particle map is surface-rendered and radially colored according to the scale bar, with the icosahedral symmetry axes labeled. (B) The magnified square area shows no openings at the twofold axis and the quasi-threefold axis, as highlighted by the lower and upper black circles, respectively. (C) The quality of the map (gray mesh) is illustrated by the fit of the Cα backbone in the separated β strands of the VP1 protein (blue wire), as well as the bulky side chains of Tyr¹¹⁵⁷, Trp²⁰⁷⁸, and Phe³²²⁰ (using the naming convention where residues in VP1, VP2, VP3, and VP4 are numbered sequentially, starting with 1001, 2001, 3001, and 4001, respectively).