Molecular pharmacology profile of VU0549108 (4). A) Concentration–response curves of 4 for human hM5, as well as hM1–4 (IC50 >10 μM), n=3). At 30 μM, % Ach Min (Ave±SEM): hM1, 61±2%; hM2, 37±2%; hM3, 55±3%; hM4, 55±1%; hM5, 16±2% B) [3H]-N-methylscopolamine (NMS) competition binding (n=3) in membranes prepared from human M5-expressing cells, showing interaction (either allosteric cooperativity or orthosteric displacement; Ki=2.7 μM.