Table 1.

List of primers used for RT-pCR assays of murine and human cancer testis antigens

	Sense or antisense strand	Gene	Size (base pairs)
Murine primers 5′–3′
ACTGGAACCATCCGCACCAGC	Sense	Sperm autoantigenic protein 17 (spa 17)	448
CACGTGTCCCCGGAAGAGGGA	Antisense
CCGCGCTCCGGTCATGGAAT	Sense	Extraembryonic spermatogenesis homeobox 1 (Esx1)	392
CCCCCAGCTGAGCGTTGGAC	Antisense
GCCCTGGTAGGCAGTGGCTC	Sense	A-kinase anchor protein 4 (akap4)	329
GCCATGTTGCCCACGGCTTC	Antisense
GTAGTCACCATGCCCAGGGGT	Sense	Melanoma antigen, family B, 5 (mageb5)	269
CCACAAACAGTGGCAGGCGA	Antisense
AAGGCAGTGCTCGGAGCCAA	Sense	Melanoma antigen, family A, 4 (magea4)	194
AGCTTCCTCAGATGGGCCTTCA	Antisense
GAGCGGTGGGAACAGAAGGCG	Sense	Reproductive homeobox 5 (rhox5)	203
GAGGGGCATCTGCCTACCCCC	Antisense
ACCACAGTCCATGCCATCAC	Sense	Glyceraldehyde-3-phosphate dehydrogenase (gapdh)	452
TCCACCACCCTGTTGCTGTA	Antisense
Human primers 5′–3′
CTGAGGGGCCTGCCCAGTCT	Sense	Melanoma antigen family C, 1 (mage-C1)	346
CGCGCCAACTCGTCCACCTT	Antisense
CATGCCCAAGGCAGGCCTCC	Sense	Melanoma antigen family A, 3 (mage-A3)	343
CAAAACCCACTCATGCAGGGGT	Antisense
GAGCAGACAGGCCAACCG	Sense	Melanoma antigen family A, 4 (mage-A4)	446
AAGGACTCTGCGTCAGGC	Antisense
AGATCAGCAGAGGGGAATGGGCC	Sense	Melanoma antigen family A, 5 (mage-A5)	97
TGGGGATCCTCTGGAGCTTCCTGGT	Antisense
TCGGTGAGGAGGCAAGTCCTG	Sense	Melanoma antigen family A, 6 (mage-A6)	204
CTGGTCAGGGCAACAGGCGG	Antisense
CAGGGCTGAATGGATGCTGCAGA	Sense	Cancer/testis antigen 1A/B (ny-eso-1)	332
GCGCCTCTGCCCTGAGGGAGG	Antisense
TGGGATCATGTTGTTGGCCCTGG	Sense	Sperm acrosome associated 3 (sllp1)	187
AGCAGCTGCGTTGAAACCGC	Antisense
TGAAGGGCTGACACGCGAGA	Sense	Sperm surface protein Sp17 (sp17)	322
TCCCCGGAAGGCAGCTTGGAT	Antisense
GCAGTCAAGGCTGTAGGAGGGC	Sense	A-kinase anchoring protein 4 (akap4)	190
TGCACACACCCCTGTGGCTG	Antisense
ATTGCCCTCAACGACCACTTTG	Sense	Glyceraldehyde-3-phosphate dehydrogenase (gapdh)	264
TTGATGGTACATGACAAGGTGCGG	Antisense