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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2016 May 27;97:136–147. doi: 10.1016/j.freeradbiomed.2016.05.013

Figure 2.

Figure 2

The effect of hydropersulfide formation on recoverable activity of NEM treated enzyme followed by DTT reduction. Recombinant CD148 was treated with or without DTNB followed by Na2S to generate the persulfide or the reduced thiol respectively. Proteins were then treated with NEM followed by DTT. Phosphatase activity was then measured using FDP and normalized at 100% to the sample where only DTT was used. These results are the normalized averages from 4 separate experiments. Error bars represent standard deviation.