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Detection of NEM-SH (M.W. = 159, retention time 10.389 min.) via select ion monitoring at m/z of 159 in (upper left) Protein-SSH sample treated with NEM and then DTT, (upper right) standard sample made via reaction of H2S with NEM, (lower left) Protein-SH treated with NEM and DTT, (lower right) Protein-SH solution treated with DTT.
