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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2016 May 27;97:136–147. doi: 10.1016/j.freeradbiomed.2016.05.013

Table 1.

Effect of Hydropersulfide Formation on Electrophilic and Oxidative Modification: Reduction of Modified Species to the Thiol via DTT.

Sample (Oxidant) Treatment Conditions Thiol Production1,2
Control

  1. reduced with DTT

  2. thiol analysis with DTNB

 100%
Thiol (HNO)

  1. reduced with DTT

  2. treated with HNO

  3. reduced with DTT

  4. thiol analysis with DTNB

 18%
Hydropersulfide (HNO)

  1. reduced with H2S,

  2. treated with HNO

  3. reduced with DTT

  4. thiol analysis with DTNB

 37%
Control

  1. reduced with DTT

  2. thiol analysis with DTNB

 100%
Thiol (H2O2)3

  1. reduced with DTT

  2. treated with H2O2

  3. reduced with DTT

  4. thiol analysis with DTNB

 39%
Hydropersulfide (H2O2)3

  1. reduced with H2S,

  2. treated with H2O2

  3. reduced with DTT

  4. thiol analysis with DTNB

 68%
1

all values normalized to the control.

2

all experiments were performed at least twice and the results of representative experiments are presented.

3

H2O2 treatment at 10 mM for 12 hrs.