Table 1.

[³H]CFT saturation binding parameters (K_d and B_max) and inhibition constant (K_i) of dopamine in competing for the binding of [³H]CFT to hDAT constructs WT, D476A and I159A with (n=4) and without Zn²⁺ treatment (n=3).

Cells	Kd of CFT, nM	Bmax, pmole/mg	DA Ki, µM
WT	21.5±3.10	2.01±0.22	4.82±0.79
D476A	29.0±7.48	1.11±0.33*	83.6±3.75 *
I159A	25.1±4.7	0.48±0.08**	26.1±3.88 *
	+ 10 µM ZnCl2
WT	6.85±1.01 #	1.28±0.20	67.4±5.49 #
D476A	5.65±1.91 #	1.26±0.02	91.8±21.6
I159A	11.39±1.14 #	0.80±0.21	29.73±5.86

^*: P<0.05 vs WT (One-way ANOVA with post-hoc Dunnett’s test)

^#: P<0.05 vs without Zn²⁺ (Student’s t-test)