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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Neurochem. 2016 Jul 14;138(5):694–699. doi: 10.1111/jnc.13704

Table 2.

Kinetic properties of [3H]dopamine uptake for WT, D476A and I159A stably expressed in LLC-PK1 cells (n=3).

Cells Km, µM Vmax,pmole/mg/min kcat, min−1 k2, min−1 k3, min−1
WT 0.76±0.06 4.54±1.18 2.26±0.63 14.3 2.68
D476A 3.27±1.27 0.29±0.10* 0.26±0.12* 6.65 0.270
I159A 1.70±0.34 0.33±0.02* 0.69±0.12* 10.6 0.738

Additional parameters, DA Ki and [3H]CFT binding Bmax, needed for these calculations were taken from Table 1.

*

: P<0.05 vs WT (One-Way Anova with post hoc Dunnett’s Method)