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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2016 Jun 23;97:292–306. doi: 10.1016/j.freeradbiomed.2016.06.025

Figure 5. α-Syn modulates the expression of TfR and ferritin in cultured RPE cells.

Figure 5

(A & B) Lysates prepared from RPE cells transfected with siRNA for α-syn, control siRNA, and non-transfected controls were analyzed by Western blotting. Probing with specific antibodies shows 90% reduction in α-syn in cells transfected with α-syn siRNA as expected (A, lane 3 vs 1, 2). Notably, knock-down of α-syn results in 4-fold reduction in TfR and 2-fold reduction in ferritin relative to controls (lane 3 vs 1, 2; B). (C & D) Over-expression of α-syn in RPE cells results in 3.5-fold increase in TfR and 3.8-fold increase in ferritin expression relative to non-transfected and vector-transfected controls (C, lane 3 vs 1, 2; D). (n=3; ***p<0.001). Reaction for β-actin served as an internal control for protein loading in panels A and C. Values are mean ± SEM of the indicated n.