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. 2016 Aug 24;6(1):56. doi: 10.1186/s13568-016-0226-8

Fig. 4.

Fig. 4

Injection of ST8/pSEndo significantly retards tumor angiogenesis. Detection of Flag-SEndo expression in the ST8/pSEndo treated tumors by western blot analysis (a) and immunohistochemical assay (b). b Composite images of a whole tumor infected with ST8/pSEndo stained to visualize bacteria (left) and Flag-SEndo (right). Immunohistochemical assay on tumor sections revealed that the therapeutic polypeptide diffused around the bacteria and some molecules have been found to be transferred to the outer rim. Quantitative RT-PCR analysis (c) and immunohistochemistry (d) to detect CD105-positive endothelial cells in tissue sections from ST8/pSGFP or ST8/pSEndo treated tumors. Scale bar 100 μm. e Angiogenesis was analyzed by quantification of haemoglobin content (n = 3). *P < 0.05