Figure 1.

The number of circulating neutrophils following S. Typhimurium challenge and Ad5-G-CSF administration. Blood samples were collected at the noted day relative to Salmonella challenge for neutrophil enumeration by flow cytometry as described in Section “Materials and Methods.” Data are reported as the mean + SEM. (A) Pigs were intranasally challenged with 1 × 10⁷ CFU of virulent S. Typhimurium UK1 strain (n = 7). Significant difference (P < 0.05) in circulating neutrophils relative to day 0 (*). (B) Pigs received a single intramuscular injection of Ad5-G-CSF followed by S. Typhimurium challenge with 1 × 10⁷ CFU of S. Typhimurium UK1 (n = 9). Significant difference (P < 0.05) in circulating neutrophils relative to day −4 (*) or day 0 (#).