Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 22;7:12417. doi: 10.1038/ncomms12417

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Representative immunostaining images of MURC, Cav1 and Cav3 in hPASMCs. Scale bar, 50 μm. (b,c) COS cells were transfected with pcDNA3.1-hMURC-HA and/or pcDNA3.1-T7-hCav1 (b) or pcDNA3.1-T7-hCav3 (c), and the cell lysates were immunoprecipitated with anti-HA and anti-T7 antibodies. (d) The in situ association of proteins in hPASMCs was assessed by the BiFC assay, which detects fluorescent signals in living cells when proteins associate with each other. Upper, hPASMCs were transfected with phmKGC-MC-hMURC and phmKGN-MC-hCav1. Lower, hPASMCs were transfected with phmKGN-MN-hMURC and phmKGC-MN-hCav3. Scale bar, 50 μm. Uncropped images of blots are shown in Supplementary Fig. 6.