Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 22;7:12417. doi: 10.1038/ncomms12417

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Immunostaining images of MURC and p115RhoGEF in hPASMCs. Scale bar, 20 μm. (b) pcDNA3.1-hMURC-HA and/or pCS2FLAG-hp115RhoGEF were cotransfected into COS cells, and the cell lysates were then immunoprecipitated with anti-HA and anti-FLAG antibodies. (c) hPASMCs were transfected with phmKGN-MC-hMURC and phmKGC-MN-hp115RhoGEF. Scale bar, 50 μm. (d) COS cells were transfected with pcDNA3.1-hMURC-FLAG and/or pcDNA3.1-hRhoA-HA and cell lysates were immunoprecipitated with anti-FLAG and anti-HA antibodies. (e) Proposed roles of the MURC-mediated activation of Rho/ROCK signalling in PASMCs. When MURC is present, it associates with Cav1, leading to reductions in the association between Cav1 and Gα13, which facilitates the interaction of Gα13 with p115RhoGEF, thereby activating the Rho/ROCK pathway. MURC also serves as a platform to compartmentalize p115RhoGEF and RhoA at caveolae. In the absence of MURC, Cav1 interacts with Gα13 and inhibits the association of Gα13 with p115RhoGEF, leading to the suppression of the Rho/ROCK pathway. Uncropped images of blots are shown in Supplementary Fig. 6.