Table 4.
Detailed list of 12 determined searching criteria for the identification of AMDases in data bases (numbering of amino acid according to the AMDase from Bordetella bronchiseptica KU1201).
| Number | Criteria | Function |
|---|---|---|
| (1) | P14 P15 | Part of large binding pocket |
| (2) | T75 S76 | Part of dioxyanion hole |
| (3) | Y126 | |
| (4) | G189 | |
| (5) | L40 | Building blocks of hydrophobic binding pocket |
| (6) | V43 | |
| (7) | Y48 | |
| (8) | V156 | |
| (9) | M159 | |
| (10) | G74 | Distinctive for AMDases instead of second active site Cys characteristic for the related racemases. |
| C188 | Protonation of postulated endiolat intermediate | |
| (11) | LMGTSLSF | Conserved sequence motifs surrounding residues that determine product’s stereoisomery (positions 72–79 and 184–191). |
| (12) | ILLSCGGL |